tra 1 81 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology tra 1 81
Tra 1 81, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tra+1+81/TRA-1-81+Antibody/pm41735622-195-51-52
Average 93 stars, based on 191 article reviews

tra 1 81 - by Bioz Stars, 2026-09

93/100 stars

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Blocking Assay:

Article Title: Derivation of primitive neural stem cells from human-induced pluripotent stem cells.
Article Snippet: Human induced pluripotent stem cells (hiPSCs) have facilitated studies on organ development and differentiation into specific lineages in in vitro systems.. Although numerous studies have focused on cellular differentiation into neural lineage using hPSCs, most studies have initially evaluated embryoid body (EB) formation, eventually yielding terminally differentiated neurons with limited proliferation potential.. This study aimed to establish human primitive neural stem cells (pNSCs) from exogene-free hiPSCs without EB formation.

Incubation:

Article Title: Transplantation of a 3D-printed tracheal graft combined with iPS cell-derived MSCs and chondrocytes
Article Snippet: .. The primary antibodies, anti-Oct4 (1:100; Santa Cruz Biotechnology, CA, USA), anti-Nanog (1:100; Santa Cruz Biotechnology, CA, USA), anti-TRA-1-81(1:100; Santa Cruz Biotechnology, CA, USA), and anti-stage-specific embryonic antigen 1(SSEA1) (1:100; Santa Cruz Biotechnology, CA, USA) were diluted in antibody diluent solution (Invitrogen, CA, USA) and samples were incubated at 4 °C overnight. .. Suitable secondary antibodies (donkey anti-rabbit Alexa Fluor 488, goat anti-mouse Alexa Fluor 555, and goat anti-rabbit Alexa Fluor 555; all from Invitrogen, CA, USA) were used, and 4′-6-diamidino-2-phenylindole (DAPI) (1:2000; Thermo Fisher, USA) was used for counterstaining.

Article Title: A three-stage differentiation method for generating induced mesenchymal stem cells from human pluripotent stem cells via gelatin-based screening.
Article Snippet: a Guangdong Engineering Research Center for antibody drugs and immunoassays, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong Province 510632, PR China b Guangzhou Future Homo sapiens Institute of Biomedicine and Health, Guangzhou 510700, PR China c Guangzhou Regenerative Medicine Research Center, Future Homo sapiens Institute of Regenerative Medicine Co. , Ltd, Guangzhou 510700, PR China d Guangzhou Santaicreeon Biopharmaceutical Co., Ltd, Guangzhou 510700, PR China e Research Center for Development and Regeneration , Guangzhou Institutes of Biomedicine and Science, Chinese Academic and Sciences, Guangzhou 510530, China

Article Title: Simplification of culture conditions and feeder-free expansion of bovine embryonic stem cells
Article Snippet: After fixation, cells were permeabilized and blocked with 0.3% Triton-X100 (T8787, Sigma) and 3% v/v of Normal Donkey Serum (NDS, D9663, Sigma) in Dulbecco’s Phosphate-Buffered Saline (DPBS, 14040174, Gibco) for 30 min at room temperature. .. Cells were then incubated for 1 h at room temperature with the following primary antibodies: anti-POU5F1 (1:300; sc-8628, Santa Cruz Biotechnology), anti-SOX2 (1:300; NU579-UC, Biogenex), anti-CDX2 (1:300; MU392A-UC, Biogenex), anti-GATA6 (1:300; sc-9055; Santa Cruz Biotechnology), anti-SSEA1 (5 μg/mL; MC-480; Developmental Studies Hybridoma Bank), anti-SSEA4 (5 μg/mL; MC-813-70; Developmental Studies Hybridoma Bank), anti-TRA-1-60 (1:250; sc-21705; Santa Cruz Biotechnology) and anti-TRA-1-81 (1:250; sc-21706; Santa Cruz Biotechnology) in DPBS supplemented with 0.3% Triton-X100 and 1% v/v of NDS. .. Secondary antibody incubation was performed in the dark for 1 h at room temperature with 1:500 fluorescently labeled donkey anti-rabbit IgG Alexa Fluor 488 antibody (A-21206, Invitrogen), donkey anti-mouse IgG Alexa Fluor 568 antibody (A-21043, Invitrogen), donkey anti-goat IgG Alexa Fluor 568 antibody (A-11057, Invitrogen), and donkey anti-mouse IgG Cy3 antibody (715-165-151, Jackson ImmunoResearch).

Article Title: Chlorogenic acid: A potent molecule that protects cardiomyocytes from TNF‐α–induced injury via inhibiting NF‐κB and JNK signals
Article Snippet: .. Cells were blocked with 5% goat serum and 5% bovine serum albumin (BSA) at room temperature, and then incubated with primary antibodies: rabbit polyclonal anti‐OCT4 (1:100; Santa Cruz Biotechnology; sc‐9081), mouse monoclonal anti‐TRA‐1‐81 (1:100; Santa Cruz Biotechnology; sc‐21706), mouse monoclonal anticardiac troponin T (cTnT) (1:100; Abcam; Ab8295) and rabbit polyclonal anti‐α‐actinin (1:100; Abcam; Ab137346) at 4°C overnight. .. After washing with PBS, the slices were incubated with secondary antibodies: chicken antirabbit IgG Alexa Fluor 594 (1:200; Invitrogen; A21442), chicken antimouse IgG Alexa Fluor 488 (1:200; Invitrogen; A21200), goat antirabbit IgG Alexa Fluor 488 (1:200; Invitrogen; A32731) and goat antimouse IgG Alexa Fluor 594 (1:200; Invitrogen; A21145) at room temperature for 1 hour.

Article Title: Simplification of culture conditions and feeder-free expansion of bovine embryonic stem cells.
Article Snippet: After fixation, cells were permeabilized and blocked with 0.3% Triton-X100 (T8787, Sigma) and 3% v/v of Normal Donkey Serum (NDS, D9663, Sigma) in Dulbecco’s Phosphate-Buffered Saline (DPBS, 14040174, Gibco) for 30 min at room temperature. .. Cells were then incubated for 1 h at room temperature with the following primary antibodies: anti-POU5F1 (1:300; sc-8628, Santa Cruz Biotechnology), anti-SOX2 (1:300; NU579-UC, Biogenex), antiCDX2 (1:300; MU392A-UC, Biogenex), anti-GATA6 (1:300; sc-9055; Santa Cruz Biotechnology), anti-SSEA1 (5 μg/mL; MC-480; Developmental Studies Hybridoma Bank), anti-SSEA4 (5 μg/mL; MC-813-70; Developmental Studies Hybridoma Bank), anti-TRA-1-60 (1:250; sc-21705; Santa Cruz Biotechnology) and anti-TRA-1-81 (1:250; sc-21706; Santa Cruz Biotechnology) in DPBS supplemented with 0.3% Triton-X100 and 1% v/v of NDS. .. Secondary antibody incubation was performed in the dark for 1 h at room temperature with 1:500 fluorescently labeled donkey anti-rabbit IgG Alexa Fluor 488 antibody (A-21206, Invitrogen), donkey anti-mouse IgG Alexa Fluor 568 antibody (A-21043, Invitrogen), donkey anti-goat IgG Alexa Fluor 568 antibody (A-11057, Invitrogen), and donkey anti-mouse IgG Cy3 antibody (715-165-151, Jackson ImmunoResearch).

Flow Cytometry:

Article Title: Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells.
Article Snippet: .. Flow cytometry and immunofluorescence analyses were employed to examine human pluripotency markers with the following antibodies: anti-Oct4 (130-105- 606; Miltenyi Biotec, Germany), anti-SSEA4 (sc-21704; Santa Cruz, USA), anti-Tra-1-60 (sc-21705; Santa Cruz, USA), anti-Tra-1-81 (sc-21706; Santa Cruz, USA), antiIgM-PE (sc-3768; Santa Cruz, USA), and anti-IgG3-PE (sc-3767; Santa Cruz, USA). ..

Immunofluorescence:

other:

Article Title: Establishment of feline embryonic stem cells from the inner cell mass of blastocysts produced in vitro
Article Snippet: Anti-Tra-1-81 , Mouse , Sc-21706 , TRA-1-80 , Monoclonal , 1:100 , Santa cruz biotechnology.

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